信号转导与转录活化因子3 在儿童颞叶局灶性皮层发育不良IIIa 型中的表达

doi:10.3969 j.issn.1000-3606.2014.09.015

临床儿科杂志 ›› 2014, Vol. 32 ›› Issue (9): 859-.doi: 10.3969 j.issn.1000-3606.2014.09.015

信号转导与转录活化因子3 在儿童颞叶局灶性皮层发育不良IIIa 型中的表达

王江娅¹, 李文玲², 武江², 娄燕²

1. 河北医科大学研究生学院( 河北石家庄　050017)；2. 河北省人民医院( 河北石家庄　050051)

收稿日期:2014-09-15 出版日期:2014-09-15 发布日期:2014-09-15
通讯作者: 娄燕 E-mail:13231195903@163.com

Expression of STAT3 in children with focal cortical dysplasias type IIIa

WANG Jiangya¹, LI Wenling², WU JIANG²,LOU Yan³

1.Hebei Medical University, Shijiazhuang 050017, Hebei, China; 2. Hebei General Hospital, Shijiazhuang 050051, Hebei, China

Received:2014-09-15 Online:2014-09-15 Published:2014-09-15

摘要/Abstract

摘要： 目的　探讨信号转导与转录活化因子3(STAT3)在儿童难治性癫痫相关性局灶性皮层发育不良(focal cortical dysplasias，FCD)IIIa型中的表达情况。方法　选取26例FCD IIIa型患儿的颞叶皮层组织和5例颅内减压或死亡患儿的颞叶组织，分别通过免疫组化、免疫荧光和蛋白免疫印迹方法，检测并比较脑组织中STAT3、P-STAT3(ser727)的表达水平。结果　P-STAT3(ser727)在FCDIIIa患儿颞叶皮层中表达较对照组增加，两组间差异有统计学意义(P<0.01)；两张连续切片分别做P-STAT3(ser727)的DAB显色和GFAP免疫荧光，证实P-STAT3阳性细胞为星形胶质细胞。结论　活化的STAT3可能参与FCDIIIa型的发病机制，并推测可能通过介导星形胶质细胞增生途径实现。

Abstract: 　Objectives　To detect the expression of STAT3 in children with FCD (focal cortical dysplasias) type IIIa. Methods The expression of STAT3 and P-STAT3 (ser727) were determined in temporal lobe specimens from 26 children with FCD type IIIa and from 5 normal control by immunohistochemistry, immunofluorescence and Western blot. Results The expression of P-STAT3 (ser727) was obviously higher in FCD type IIIa than in control group. P-STAT3 (ser727) was located mainly in the nucleus of astrocytes. Conclusions Activated STAT3 might contribute to the patholgenesis of FCD type IIIa by mediating proliferation of astrocytes.

王江娅, 李文玲, 武江, 娄燕. 信号转导与转录活化因子3 在儿童颞叶局灶性皮层发育不良IIIa 型中的表达[J]. 临床儿科杂志, 2014, 32(9): 859-.

WANG Jiangya, LI Wenling, WU JIANG,LOU Yan. Expression of STAT3 in children with focal cortical dysplasias type IIIa[J]. , 2014, 32(9): 859-.

信号转导与转录活化因子3 在儿童颞叶局灶性皮层发育不良IIIa 型中的表达

Expression of STAT3 in children with focal cortical dysplasias type IIIa

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